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Several studies have shown that different mouse strains display a high variability in the degree of neovascularization [ 13 , 14 ]. PAD is caused by atherosclerotic plaque progression which leads to the occlusion of peripheral arteries and results in claudication intermittens, or in more severe cases, in critical limb ischemia. The researchers should recognize the variants to be able to choose the most suitable model for their experiments, and they should describe the method and the used anatomical landmarks in order to make comparisons possible among studies. The blood perfusion values are measured per pixel and the region of interest can be indicated on the images by manually drawing these regions of interest. In this model, limb ischemia is induced by ligation, electrocoagulation, or by applying an ameroid constrictor to occlude the femoral artery. Lysine acetyltransferase PCAF is a key regulator of arteriogenesis. For the current review, this method is less relevant, since the induced ischemia is for such a short period that no effects on neovascularization are observed. Therefore, ketamine and alpha-agonist combinations are not suitable for studies of vascular smooth muscle cell rich vasculature [ 23 ].

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