Flow cytometry was performed as described Logan et al. Next, we studied the growth-regulating properties of DP-4 by introducing DP-4 either alone or together with E2F-1 into U2OS cells, which represents a cell-type that has been used extensively to study the cell-cycle-regulating properties of E2F subunits. Immunostaining indicated that DP-4 is primarily located in the nuclei of transfected cells Figure 2B. Access through your institution. Our results indicate that DP-4 exhibits certain properties in common with other DP proteins but also differences that establish it as a unique and interesting member of the family. DP subunits are heterodimeric partner proteins for E2F subunits. ChIP performed on the albumin promoter using primers as previously described Zhu et al. We investigated the transcription regulating properties of DP-4 on a panel of E2F responsive-promoter constructs. Thus, E2F activity is intimately connected with proliferation control, and its deregulation contributes significantly to the abnormal growth of tumour cells.
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